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2 edition of Studies on the isolation of Listeria monocytogenes from experimentally infected mice. found in the catalog.

Studies on the isolation of Listeria monocytogenes from experimentally infected mice.

Thomas Ross Wilkinson

Studies on the isolation of Listeria monocytogenes from experimentally infected mice.

by Thomas Ross Wilkinson

  • 159 Want to read
  • 26 Currently reading

Published .
Written in English

    Subjects:
  • Listeria monocytogenes.

  • The Physical Object
    Paginationviii, 59 l.
    Number of Pages59
    ID Numbers
    Open LibraryOL16721784M

    D.R. Kalorey's 47 research works with citations and 2, reads, including: Inflamosomes as Activated Molecular Platform for Engagement of Innate Immune Defences.   A Listeria monocytogenes mutant defective in bacteriophage attachment is attenuated in orally inoculated mice and impaired in enterocyte intracellular growth. Infect. Immun. 76, .

    The human pathogen L. monocytogenes is a facultatively intracellular bacterium that survives and replicates in the cytosol of many mammalian cells. The listerial metabolism, especially under intracellular conditions, is still poorly understood. Recent studies analyzed the carbon metabolism of L. monocytogenes by the 13C isotopologue perturbation method in a defined minimal medium . tion with wild-type strains of L. monocytogenes in mice (Kaufmann, ). The current study was designed primarily to assess the effectiveness of dietary vitamin E, using doses previously shown to enhance meat quality, in accelerating the gut clearance of L. monocytogenes in experimentally infected adult turkeys. Secondarily, we monitored CD4.

    Fluorescent Listeria strains have been used previously to study specific infected host cell populations in the central nervous system of infected mice. However, this study was limited to early time points due to the use of multicopy plasmids. Our results show that we could detect fluorescent L. monocytogenes in spleens after 72 h of infection. Female CD-1 mice were injected intraperitoneally with L. monocytogenes A At 0, 10, and 20 hr after infection, groups of mice were sacrificed and the livers were removed and pooled. Oxidative phosphorylation was assayed immediately upon isolation of .


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Studies on the isolation of Listeria monocytogenes from experimentally infected mice by Thomas Ross Wilkinson Download PDF EPUB FB2

Studies of listeria monocytogenes (bacterium) in some selected local government areas of plateau state, nigeria.

home botany project topics and materials studies of listeria monocytogenes (bacterium) in some selected local government areas of plateau state, nigeria. project file details. Listeria monocytogenes cause foodborne disease in humans that ranges in severity from mild, self-limiting gastroenteritis to life-threatening systemic infections of the blood, brain, or placenta.

The most commonly used animal model of listeriosis is intravenous infection of mice. This systemic model is highly reproducible, and thus, useful for studying cell-mediated immune responses against an Cited by: Wilkinson TR, Hall ER.

Rapid method for the isolation of Listeria monocytogenes from experimentally infected mice. Appl Microbiol. Jan; 21 (1)– [PMC free article] ZINK A, de MELLO GC, BURKHART RL.

Listeriosis; field and laboratory studies, and aureomycin activity. Am J Vet Res. Jul; 12 (44)–Cited by:   In a study of central nervous system involvement in experimental listeriosis 27 Swiss CD1 mice were inoculated subcutaneously withListeria ic infection developed, as shown by the isolation ofListeria monocytogenes and histopathological lesions in the spleen and liver.

In the central nervous system a mixed inflammatory infiltration in the ventricular system, Cited by: Listeria monocytogenes was successfully isolated from experimentally infected mice by placing homogenized tissues in phosphate-buffered saline (PBS), trypsin, peptone, or pepsin followed by incubation at 37 C for 24 hr.

A larger number of Listeria isolates were recovered from the trypsin or PBS splenic homogenate suspensions incubated at 37 C for 2 hr. than from the other diluent by: 1.

Listeria monocytogenes is a Gram-positive facultative intracellular bacterium that is widely used to characterize bacterial pathogenesis and host immunity. Here, we describe a set of basic methods and techniques to infect mice with L. monocytogenes, measure bacterial load in tissues, and analyze immune cell subsets responding to infection in.

Chlorella vulgaris extract (CVE) was examined for its chelating effects on the myelosuppression induced by lead in Listeria monocytogenes-infected mice. The reduction in the number of bone marrow granulocyte-macrophage progenitors (CFU-GM) observed after the infection.

In the present study, we investigated the pathogenesis of systemic infection by the food-borne isolate Scott A in an intragastric (i.g.) mouse challenge model. We found that the severity of infection with L. monocytogenes Scott A was increased in mice made neutropenic by administration of monoclonal antibody RBC5.

INTRODUCTION. The Gram-positive pathogenic bacterium Listeria monocytogenes is a causative agent of severe foodborne infection in humans and a wide range of domestic and wild animals (Gray and Killinger ; Vázquez-Boland et al.

; Oevermann et al. ).Listeria monocytogenes is a ubiquitous bacterium and has been isolated from soil, plant debris and sewage (Weis and Seeliger. Mice. 8-week-old female NMRI mice were obtained in a pathogen free state (Zentralin­stitut fur Versuchstierzucht Hannover, Germany).

The mice were kept under conventional conditions and fed with pellets and water ad libitum. Mouse infection model. Mice were infected intravenously with 2 x L. monocytogenes in ml of PBS. Here we performed intra-testicular bacterial infection with Listeria monocytogenes (L.

monocytogenes) as a model of experimental orchitis. 12 We. Listeria monocytogenes is a Gram-positive, facultative intracellular bacterium that can contaminate food which, upon ingestion, can result in infection of a wide range of animals, including livestock and humans (Vázquez-Boland et al., ).The severity and extent of L.

monocytogenes infection is determined by the virulence of the bacterial strain and the host’s immune status. Listeria monocytogenes consists of at least 4 evolutionary lineages (I, II, III, and IV) with different but overlapping ecological niches.

Most L. monocytogenes isolates seem to belong to lineages I and II, which harbor the serotypes more commonly associated with human clinical cases, including serotype 1/2a (lineage II) and serotypes 1/2b and 4b (lineage I).

Listeria monocytogenes is a saprophytic, gram-positive rod, ubiquitously distributed in the environment. It is the etiologic agent of listeriosis, a food-borne disease affecting humans and a variety of vertebrates ().Listeriosis occurs primarily in immunocompromised individuals, causing septicemia, meningitis, and meningoencephalitis, and in pregnant women, causing spontaneous abortion.

The positive isolation of L. monocytogenes was verified biochemically as described by Seeliger and Jones (25) and serologically byusing tube agglutination with Listeria type OIantiserum (Difco). Preparation ofantigen.

monocytogenes (1-liter culture) grown in tryptose broth (Difco) at 20°C for 18 h was harvestedbycentrifugation at 5, x. Abstract. Listeria monocytogenes is a Gram-positive facultative intracellular bacterium that is widely used to characterize bacterial pathogenesis and host immunity.

Here, we describe a set of basic methods and techniques to infect mice with togenes, measure bacterial load in tissues, and analyze immune cell subsets responding to infection in the spleen and liver.

Knock-out mice were used to study pathways contributing to increase of IFNβ and IFNα in the serum of mice infected with L. monocytogenes. We established 24 h post intraperitoneal (i.p.) injection of bacteria as the point of maximum IFN-I induction in preliminary experiments (data not shown); therefore, IFNβ and IFNα were measured 24 h after.

There have been no studies on the susceptibility and host immune responses to an intranasal infection with Listeria monocytogenes. In this study, we compared the susceptibilities and cytokine responses between intranasal and intravenous infections with L.

monocytogenes in mice. Moreover, we compared efficiency of acquisition of host resistance to L. monocytogenes infection between. Listeria monocytogenes is a facultative intracellular pathogen that induces a cytosolic signaling cascade resulting in expression of interferon (IFN)-β.

Although type I IFNs are critical in viral defense, their role in immunity to bacterial pathogens is much less clear.

In this study, we addressed the role of type I IFNs by examining the infection of L. monocytogenes in BALB/c mice lacking. It could be argued that we understand the immune response to infection with Listeria monocytogenes better than the immunity elicited by any other bacteria.

monocytogenes are Gram-positive bacteria that are genetically tractable and easy to cultivate in vitro, and the mouse model of intravenous (i.v.) inoculation is highly reproducible. For these reasons, immunologists frequently use the. Subacute and chronic Listeria monocytogenes infections were produced in mice by i/p injection of million organisms.

Bacteriological examinations after s/c treatment with 5 different drugs revealed that tetracycline was best for ridding mice of listeria, followed by erythromycin and a procaine penicillin-dihydro-streptomycin combination. All mice given 2 courses of treatment with.Listeria monocytogenes Infection from Foods Prepared in a Commercial Establishment: A Case-Control Study of Potential Sources of Sporadic Illness in the United States.

Clinical Infectious Diseases, Vol. 44, Issue. 4, p. Abstract. Listeria monocytogenes is a food-borne pathogen that must cross the intestinal epithelial barrier to reach its target organs.

We have investigated the importance of M cells in translocation using an experimental mouse model and a novel, recently described in vitro co-culture system that mimics the follicle-associated epithelium (FAE).